Reporter
Part:BBa_J100069:Design
Designed by: Rebecca Evans Group: Campbell M Lab (2012-06-28)
Superfolder GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 750 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 751
Illegal PstI site found at 765
Illegal NotI site found at 7
Illegal NotI site found at 758 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 751 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 751
Illegal PstI site found at 765
Illegal AgeI site found at 54 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 23
Illegal SapI.rc site found at 42
Design Notes
Designed to encode for superfolder GFP, specifically codon optimized for "E. coli".