Reporter

Part:BBa_J100069:Design

Designed by: Rebecca Evans   Group: Campbell M Lab   (2012-06-28)

Superfolder GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 751
    Illegal PstI site found at 765
    Illegal NotI site found at 7
    Illegal NotI site found at 758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 751
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 751
    Illegal PstI site found at 765
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal SapI.rc site found at 42


Design Notes

Designed to encode for superfolder GFP, specifically codon optimized for "E. coli".


Source

References